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Axion CytoSMART Exact細胞(bao)(bao)(bao)計數(shu)儀，是(shi)一款(kuan)荷蘭進口全自動(dong)細胞(bao)(bao)(bao)計數(shu)儀器，無論是(shi)在明場還是(shi)熒光通道下(xia)，都可(ke)以高效、準(zhun)確(que)地評(ping)估2D/3D細胞(bao)(bao)(bao)濃度和(he)活力，獲得精(jing)準(zhun)可(ke)重復的實驗(yan)結果。由于使用了新型(xing)的光學和(he)圖像分析軟件技術，可(ke)實現自動(dong)對焦，3s完成細胞(bao)(bao)(bao)計數(shu)，可(ke)以區分細胞(bao)(bao)(bao)及其碎片、識別出細胞(bao)(bao)(bao)團(tuan)中(zhong)的個體、計算類器官的大(da)小(xiao)和(he)數(shu)量等。



 
CytoSMART Exact細(xi)胞計數儀(yi)應用(yong)

1、各類細胞的全自動細胞計數、活率分析、細胞大小分析：

明場(chang)利用(yong)臺盼藍(lan)染(ran)(ran)色(se)劑、或雙熒光通道使(shi)用(yong)綠色(se)/紅色(se)熒光染(ran)(ran)色(se)劑（AO/PI雙染(ran)(ran)），可(ke)以(yi)計算細(xi)(xi)(xi)胞(bao)(bao)總(zong)數（細(xi)(xi)(xi)胞(bao)(bao)數/mL）、活細(xi)(xi)(xi)胞(bao)(bao)及(ji)死(si)細(xi)(xi)(xi)胞(bao)(bao)數量（細(xi)(xi)(xi)胞(bao)(bao)數/mL）、細(xi)(xi)(xi)胞(bao)(bao)活力(li)(%)、活細(xi)(xi)(xi)胞(bao)(bao)及(ji)死(si)細(xi)(xi)(xi)胞(bao)(bao)的大小(xiao)（μm）。可(ke)實現從細(xi)(xi)(xi)胞(bao)(bao)團中分辨單個細(xi)(xi)(xi)胞(bao)(bao)，通過(guo)細(xi)(xi)(xi)胞(bao)(bao)大小(xiao)門控設定來選擇細(xi)(xi)(xi)胞(bao)(bao)亞群，了解樣本(ben)內細(xi)(xi)(xi)胞(bao)(bao)大小(xiao)分布情(qing)況。
 

 

2、球形和不規則形狀類器官的計數、大小、群體分布分析：

類(lei)(lei)(lei)(lei)(lei)器(qi)官(guan)(guan)(guan)計數模(mo)塊采用(yong)了新型AI分析(xi)算(suan)法，能夠快(kuai)速(su)精準地(di)識(shi)別不(bu)同(tong)形狀的(de)(de)類(lei)(lei)(lei)(lei)(lei)器(qi)官(guan)(guan)(guan)，在(zai)計算(suan)樣(yang)(yang)本(ben)數量(liang)的(de)(de)同(tong)時，給出大小(xiao)分布。軟件(jian)通過“總覽”、“類(lei)(lei)(lei)(lei)(lei)器(qi)官(guan)(guan)(guan)濃(nong)度(du)”、“類(lei)(lei)(lei)(lei)(lei)器(qi)官(guan)(guan)(guan)面積”，從不(bu)同(tong)角度(du)和時間(jian)維度(du)來展示受(shou)測樣(yang)(yang)本(ben)的(de)(de)特征(zheng)。可(ke)以選擇手動或自動設定(ding)細胞(bao)大小(xiao)門控，了解樣(yang)(yang)本(ben)中類(lei)(lei)(lei)(lei)(lei)器(qi)官(guan)(guan)(guan)在(zai)不(bu)同(tong)時間(jian)點的(de)(de)濃(nong)度(du)及平均大小(xiao)，或是不(bu)同(tong)尺寸類(lei)(lei)(lei)(lei)(lei)器(qi)官(guan)(guan)(guan)在(zai)數量(liang)上的(de)(de)分布規律，將多(duo)個時間(jian)點的(de)(de)分析(xi)數據歸(gui)納到一起比較，還可(ke)以掌握類(lei)(lei)(lei)(lei)(lei)器(qi)官(guan)(guan)(guan)數量(liang)變化對于樣(yang)(yang)本(ben)總體細胞(bao)濃(nong)度(du)的(de)(de)影響(xiang)。




 

CytoSMART Exact細胞計數儀優勢

1、快速計數，高準確率

全自動對焦，3s完成細胞(bao)計(ji)數；采用高(gao)(gao)倍率、高(gao)(gao)對比度光學組(zu)件，640萬CMOS成像，高(gao)(gao)分辨率有(you)效(xiao)區分細胞(bao)和(he)碎片；更大(da)(da)視(shi)野(ye)，可(ke)實(shi)現同一樣本(ben)8個區域拍照取(qu)均值；使(shi)用電腦鏈接國內云端服務器(qi)，進行(xing)圖像分析(xi)和(he)儲(chu)存，通過(guo)創(chuang)新的機(ji)器(qi)學習軟件，大(da)(da)幅提(ti)高(gao)(gao)了(le)實(shi)驗的準(zhun)確性和(he)可(ke)重(zhong)復(fu)性，并有(you)效(xiao)降(jiang)低了(le)人工計(ji)數時不(bu)同人員操作差異對結(jie)果的影響。

2、熒光計數，細胞亞群分析

除通過(guo)明(ming)場獲得細胞(bao)總(zong)數(shu)量(liang)(liang)、活力外，還可通過(guo)紅色熒光、綠色熒光通道計算(suan)樣本中特定類群細胞(bao)的(de)數(shu)量(liang)(liang)。

3、使用方便

操作簡單、無需(xu)安裝、無需(xu)校準、無需(xu)維護，任何實(shi)驗室都(dou)能快速(su)上手開始實(shi)驗。

4、兼容各品牌細胞計數板

兼容市面(mian)上各類透明、深(shen)度為0.1mm計數板(ban)，不(bu)論(lun)塑料、還是玻璃材質，不(bu)論(lun)有無記數格(ge)，不(bu)論(lun)有幾個計數腔室，不(bu)論(lun)是重(zhong)復使用還是一次性計數板(ban)，均(jun)可用于CytoSMART Exact。

 

參考文獻

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